Artigo
Cultivo in vitro da bananeira Prata Anã clone Gorutuba, em meio líquido, agitado e estacionário
Autor
Costa, Annanda Mendes
Faria, Renata Aparecida Neres
Londe, Luciana Nogueira
Ribeiro, Emerson Brito
Damascena, Nayara de Souza
Institución
Resumen
O objetivo deste trabalho foi avaliar o cultivo in vitro da bananeira Prata Anã (Musa spp.), clone Gorutuba, em diferentes volumes de meio líquido, nos sistemas estacionário e sob agitação, comparando-os com o cultivo tradicional em meio semissólido. O experimento foi conduzido em delineamento inteiramente casualizado, sendo um fatorial 5 x 2 + 1, com cinco volumes (10, 15, 20, 25 e 30 mL) de meio líquido e dois sistemas de cultivo (agitação e estacionário) e a testemunha (meio semissólido), com quatro repetições, sendo cada parcela constituída por um frasco com quatro explantes. O número de brotações por explante, observado pela testemunha no terceiro subcultivo, diferiu de alguns tratamentos em meio líquido. No quarto subcultivo, o número de brotações por explante da testemunha diferiu de todos os tratamentos em meio líquido e o sistema de cultivo sob agitação favoreceu um número de brotações por explante significativamente superior ao obtido pelo sistema estacionário. Na fase de alongamento e enraizamento, os resultados verificados em meio líquido foram melhores que os da testemunha. Comparando-se os dois sistemas de cultivo em meio líquido, constatou-se melhor crescimento das plântulas que permaneceram sob agitação com o volume de 30 mL. O cultivo em meio semissólido é mais eficiente na fase de multiplicação in vitro; nas fases de alongamento e de enraizamento, o melhor sistema a ser adotado é o cultivo em meio líquido sob agitação, com o volume de 30 mL The aim of this study was to evaluate in vitro cultivation of banana Prata Anã clone Gorutuba in differents volumes of liquid medium in the stationary system and under agitation, comparing with the traditional cultivation in semisolid medium. The experiment was conducted in a completely randomized design with factorial 5 x 2 he aim of this study was to evaluate in vitro cultivation of banana Prata Anã clone Gorutuba in differents volumes of liquid medium in the stationary system and under agitation, comparing with the traditional cultivation in semisolid medium. The experiment was conducted in a completely randomized design with factorial 5 x 2 + 1, with five volumes (10, 15, 20, 25 and 30 mL) of liquid medium and two cropping systems (agitation and stationary) and control (semisolid medium), with 4 replicates, each plot had a bottle with 4 explants. The number of shoots/explant observed by the control on the third subculture differs from some treatments in liquid medium. In the fourth subculture, the number of shoots/explant of the control differed from all treatments in liquid medium, and cultivation under agitation system favored a number of shoots/explant significantly higher than that obtained by the stationary system. In the elongation and rooting phases, the results obtained in liquid medium, they were better than the control. Comparing the two systems of cultivation in liquid medium, we noted greater growth for plantlets which remained under agitation with the volume of 30ml. The cultivation in semisolid medium is more efficient in vitro multiplication phase, and in the phase of elongation and rooting the best system to be adopted is the cultivation in liquid medium under agitation to 30 mL+ 1, with five volumes (10, 15, 20, 25 and 30 mL) of liquid medium and two cropping systems (agitation and stationary) and control (semisolid medium), with 4 replicates, each plot had a bottle with 4 explants. The number of shoots/explant observed by the control on the third subculture differs from some treatments in liquid medium. In the fourth subculture, the number of shoots/explant of the control differed from all treatments in liquid medium, and cultivation under agitation system favored a number of shoots/explant significantly higher than that obtained by the stationary system. In the elongation and rooting phases, the results obtained in liquid medium, they were better than the control. Comparing the two systems of cultivation in liquid medium, we noted greater growth for plantlets which remained under agitation with the volume of 30ml. The cultivation in semisolid medium is more efficient in vitro multiplication phase, and in the phase of elongation and rooting the best system to be adopted is the cultivation in liquid medium under agitation to 30 mL