Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFusinatto, L. A.
dc.creatorLopes, S.
dc.creatorSilva-Ferreira, A.
dc.creatorAlexandrino, J.
dc.creatorHaddad, C. F B
dc.creatorRocha, C. F D
dc.creatorSequeira, F.
dc.date2014-05-27T11:28:12Z
dc.date2016-10-25T18:42:53Z
dc.date2014-05-27T11:28:12Z
dc.date2016-10-25T18:42:53Z
dc.date2013-01-24
dc.date.accessioned2017-04-06T02:10:44Z
dc.date.available2017-04-06T02:10:44Z
dc.identifierGenetics and Molecular Research, v. 12, n. 1, p. 230-234, 2013.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/74416
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74416
dc.identifier10.4238/2013.January.24.15
dc.identifierWOS:000318864400025
dc.identifier2-s2.0-84872892433.pdf
dc.identifier2-s2.0-84872892433
dc.identifierhttp://dx.doi.org/10.4238/2013.January.24.15
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895182
dc.descriptionThe new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population (Reserva Ecológica de Guapiaçu, REGUA) locatedin the central region of the Rio de Janeiro State, in Brazil. The mean number of alleles per locus ranged from 3 to 12. Observed and expected heterozygosities ranged from 0.095 to 0.905 and from 0.094 to 0.904, respectively. After using the Bonferroni correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci but deviations for Hardy-Weinberg equilibrium were found in 4 loci. We found no evidence for allele dropouts or stuttering, but we detected the presence of null alleles at loci Eb10 and Eb36. These markers will be useful for analyses of fine-scale population structure and determination of relative effects of habitat loss and fragmentation on population genetic variability within species. © FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject454 sequencing
dc.subjectAmphibians
dc.subjectAtlantic forest
dc.subjectEuparkerella brasiliensis
dc.subjectMicrosatellites
dc.subjectanimal tissue
dc.subjectAnura
dc.subjectBrazil
dc.subjectendemic species
dc.subjectgene isolation
dc.subjectgene locus
dc.subjectgene sequence
dc.subjectgenetic variability
dc.subjectgenotype
dc.subjectgeographical variation (species)
dc.subjectheterozygosity
dc.subjectmicrosatellite marker
dc.subjectmultiplex polymerase chain reaction
dc.subjectNeotropics
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectnull allele
dc.subjectprocess development
dc.subjectprocess optimization
dc.subjectpyrosequencing
dc.titleDevelopment of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing
dc.typeOtro


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