The importance of hormone receptor analysis in osteosarcoma cells growth submitted to treatment with estrogen in association with thyroid hormone

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSaraiva, Patricia Pinto
dc.creatorTeixeira, Silvania Silva
dc.creatorConde, Sandro José
dc.creatorNogueira, Célia Regina
dc.date2014-05-27T11:22:46Z
dc.date2016-10-25T18:25:06Z
dc.date2014-05-27T11:22:46Z
dc.date2016-10-25T18:25:06Z
dc.date2008-01-01
dc.date.accessioned2017-04-06T01:29:30Z
dc.date.available2017-04-06T01:29:30Z
dc.identifierCell Biochemistry and Function, v. 26, n. 1, p. 107-110, 2008.
dc.identifier0263-6484
dc.identifier1099-0844
dc.identifierhttp://hdl.handle.net/11449/70235
dc.identifierhttp://acervodigital.unesp.br/handle/11449/70235
dc.identifier10.1002/cbf.1408
dc.identifier2-s2.0-38549092231
dc.identifierhttp://dx.doi.org/10.1002/cbf.1408
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891376
dc.descriptionBone tumor incidence in women peaks at age 50-60, coinciding with the menopause. That estrogen (E2) and triiodothyronine (T3) interact in bone metabolism has been well established. However, few data on the action of these hormones are available. Our purpose was to determine the role of E2 and T3 in the expression of bone activity markers, namely alkaline phosphatase (AP) and receptor activator of nuclear factor κB ligand (RANKL). Two osteosarcoma cell lines: MG-63 (which has both estrogen (ER) and thyroid hormone (TR) receptors) and SaOs-29 (ER receptors only) were treated with infraphysiological E2 associated with T3 at infraphysiological, physiological, and supraphysiological concentrations. Real-time RT-PCR was used for expression analysis. Our results show that, in MG-63 cells, infraphysiological E2 associated with supraphysiological T3 increases AP expression and decreases RANKL expression, while infraphysiological E2 associated with either physiological or supraphysiological T3 decreases both AP and RANKL expression. On the other hand, in SaOs-2 cells, the same hormone combinations had no significant effect on the markers' expression. Thus, the analysis of hormone receptors was shown to be crucial for the assessment of tumor potential growth in the face of hormonal changes. Special care should be provided to patients with T3 and E2 hormone receptors that may increase tumor growth. Copyright © 2007 John Wiley & Sons, Ltd.
dc.languageeng
dc.relationCell Biochemistry and Function
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlkaline phosphatase
dc.subjectBone
dc.subjectEstrogen
dc.subjectHormone receptors
dc.subjectMG-63
dc.subjectRANKL
dc.subjectSaOs-2
dc.subjectThyroid hormone
dc.subjectalkaline phosphatase
dc.subjectestradiol
dc.subjectestrogen
dc.subjectestrogen receptor
dc.subjecthormone receptor
dc.subjectliothyronine
dc.subjectosteoclast differentiation factor
dc.subjectthyroid hormone
dc.subjectthyroid hormone receptor
dc.subjectcancer growth
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjecthormone determination
dc.subjecthormone metabolism
dc.subjecthuman
dc.subjecthuman cell
dc.subjectosteosarcoma
dc.subjectosteosarcoma cell
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectreverse transcription polymerase chain reaction
dc.subjectCell Line, Tumor
dc.subjectEstrogens
dc.subjectFemale
dc.subjectHumans
dc.subjectMiddle Aged
dc.subjectOsteosarcoma
dc.subjectReceptors, Estrogen
dc.subjectReceptors, Thyroid Hormone
dc.subjectThyroid Hormones
dc.titleThe importance of hormone receptor analysis in osteosarcoma cells growth submitted to treatment with estrogen in association with thyroid hormone
dc.typeOtro


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