Purification and biochemical characterization of two xylanases produced by Aspergillus caespitosus and their potential for kraft pulp bleaching

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSandrim, V. C.
dc.creatorRizzatti, A. C S
dc.creatorTerenzi, H. F.
dc.creatorJorge, J. A.
dc.creatorMilagres, A. M F
dc.creatorPolizeli, M. L T M
dc.date2014-05-27T11:21:18Z
dc.date2016-10-25T18:20:36Z
dc.date2014-05-27T11:21:18Z
dc.date2016-10-25T18:20:36Z
dc.date2005-04-01
dc.date.accessioned2017-04-06T01:12:47Z
dc.date.available2017-04-06T01:12:47Z
dc.identifierProcess Biochemistry, v. 40, n. 5, p. 1823-1828, 2005.
dc.identifier1359-5113
dc.identifierhttp://hdl.handle.net/11449/68172
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68172
dc.identifier10.1016/j.procbio.2004.06.061
dc.identifier2-s2.0-13844298116
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2004.06.061
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889545
dc.descriptionTwo extracellular xylanases produced by the thermotolerant fungus Aspergillus caespitosus grown in sugar cane bagasse were purified and characterized. Estimated molecular masses were 26.3 and 27 kDa (xyl I); 7.7 and 17.7 kDa (xyl II) for gel filtration and SDS-PAGE, respectively. Optimal temperature for both xylanases was 50-55°C. Optimal pH was 6.5-7.0 for xyl I, and 5.5-6.5 for xyl II. The thermostability (T half) at 55°C was 27.3 min (xyl I) and >90 min (xyl II). Xylanase activity was inhibited by several ions. β-mercaptoethanol activated 59 and 102% xyl I and xyl II activities, respectively. These enzymes preferentially hydrolyzed birchwood xylan, and the K m and V max values were 2.5 mg/ml and 1679 U/mg protein (xyl I), and 3.9 mg/ml and 113 U/mg protein (xyl II). The action of both xylanases mainly that of xyl II, on kraft pulp reduced kappa number and increased pulp viscosity. © 2004 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.relationProcess Biochemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAspergillus caespitosus
dc.subjectPulp bleaching
dc.subjectXylanases
dc.subjectBagasse
dc.subjectBiochemistry
dc.subjectBleaching
dc.subjectCharacterization
dc.subjectEthanol
dc.subjectFiltration
dc.subjectFungi
dc.subjectKraft pulp
dc.subjectPurification
dc.subjectSugar cane
dc.subjectThermal effects
dc.subjectViscosity
dc.subjectBiochemical characterization
dc.subjectGel filtration
dc.subjectMolecular masses
dc.subjectOptimal temperatures
dc.subjectXylanase activity
dc.subjectEnzymes
dc.subjectArundinaria
dc.subjectAspergillus
dc.subjectSaccharum hybrid cultivar
dc.titlePurification and biochemical characterization of two xylanases produced by Aspergillus caespitosus and their potential for kraft pulp bleaching
dc.typeOtro


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