Article
An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
Registro en:
GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 7, p. 915-917, Oct. 2003.
0074-0276
10.1590/s0074-02762003000700010
1678-8060
Autor
Guimarães, Erick Vaz
Carvalho, Laís de
Barbosa, Helene Santos
Resumen
Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.