Does DHN-Melanin Always Protect Fungi against Antifungal Drugs? The Fonsecaea/Micafungin Paradigm

dc.creatorCoelho, Rowena Alves
dc.creatorFigueiredo-Carvalho, Maria Helena Galdino
dc.creatorSilva, Juliana Vitória dos Santos
dc.creatorCorrea-Junior, Dario
dc.creatorFrases, Susana
dc.creatorZancopé-Oliveira, Rosely Maria
dc.creatorFreitas, Dayvison Francis Saraiva
dc.creatorAlmeida-Paes, Rodrigo
dc.date2023-04-24T22:47:01Z
dc.date2023-04-24T22:47:01Z
dc.date2022
dc.date.accessioned2023-09-26T23:22:19Z
dc.date.available2023-09-26T23:22:19Z
dc.identifierCOELHO, Rowena Alves et al. Does DHN-Melanin Always Protect Fungi against Antifungal Drugs? The Fonsecaea/Micafungin Paradigm. Microbiology Research, v. 13, n. 2, p. 201-209, 2022.
dc.identifier2456-7043
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57956
dc.identifier10.3390/microbiolres13020017
dc.identifier2456-7043
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889851
dc.descriptionThis research was funded by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), grant number E-26/201.441/2021 and Inova Fiocruz/VPPCB, grant number VPPCB-008-FIO-18-2-49. R.M.Z-O. was supported in part by Conselho Nacional de Desenvolvimento Científico e Tecnológico [CNPq 302796/2017-7] and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro [FAPERJ E-26/202.527/2019].
dc.descriptionSeveral human pathogenic fungi produce melanin. One of its properties during parasitism is the protection against antifungal drugs. This occurs with the agents of chromoblastomycosis, in which DHN-melanin reduces antifungal susceptibility to terbinafine and itraconazole. Since these agents are resistant to some antifungal drugs, we investigated the role of DHN-melanin on the Fonsecaea susceptibility to amphotericin B, micafungin, fluconazole, and flucytosine, drugs that usually present high minimal inhibitory concentrations (MIC) to this genus. Seven strains from three Fonsecaea human pathogenic species were treated with tricyclazole, a DHN-melanin inhibitor, and the MIC of the treated and untreated cells were compared. A survival assay was performed to confirm the alterations in the susceptibility of strains with reduced melanization, and the chitin levels of the strains were estimated by fluorescence. Tricyclazole did not affect fluconazole and flucytosine MIC, while melanin inhibition increased susceptibility to amphotericin B. Surprisingly, DHN-melanin inhibition decreased the susceptibility to micafungin. Survival assays confirmed this result on five strains. Cell wall chitin levels of the strains were not associated with the decrease in micafungin susceptibility. The results show that DHN-melanin does not have a role in the intrinsic resistance of Fonseacaea spp. to amphotericin B, fluconazole, and flucytosine, and its inhibition may promote micafungin resistance.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectAntifungal susceptibility
dc.subjectChromoblastomycosis
dc.subjectFonsecaea
dc.subjectDHN-melanin
dc.subjectMicafungin
dc.titleDoes DHN-Melanin Always Protect Fungi against Antifungal Drugs? The Fonsecaea/Micafungin Paradigm
dc.typeArticle


Este ítem pertenece a la siguiente institución