Article
Food safety evaluation: Detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry
Registro en:
NICOLICH, Rebecca S.; WERNECK-BARROSO, Eduardo; MARQUES, Marlice A. Sípoli. Food safety evaluation: Detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, v. 565, p. 97–102, 2006.
0003-2670
10.1016/j.aca.2006.01.105
Autor
Nicolich, Rebecca S.
Werneck-Barroso, Eduardo
Marques, Marlice A. Sípoli
Resumen
A simple and rapid procedure for extraction of chloramphenicol (CAP) in milk and analysis by high-performance liquid chromatography coupled with quadrupole mass spectrometry in tandem was developed. The method consisted of one step of liquid–liquid extraction using ethyl acetate and acidified water (10 mmol L−1 formic acid) and HPLC-MS/MS detection. CAP-D5 was used as internal standard. The method was validated according to Commission Decision 2002/657/EC. The calibration curves were linear, with typical r2 values higher than 0.98. Absolute recovery of CAP from milk proved to be more than 95%, however CAP-D5 absolute recovery was 75%. The method was accurate and reproducible, being successfully applied to the monitoring of CAP in milk samples obtained from the Brazilian market. Decision limit (CC ) was 0.05 ng mL−1 and detection capability (CC) was 0.09 ng mL−1. 2030-12-31