Transposon Tn1721 distribution among strains of Xylella fastidiosa

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFerreira, Lucia P
dc.creatorLemos, Eliana G.M
dc.creatorLemos, Manoel Victor F
dc.date2014-05-27T11:20:25Z
dc.date2016-10-25T18:17:42Z
dc.date2014-05-27T11:20:25Z
dc.date2016-10-25T18:17:42Z
dc.date2002-03-05
dc.date.accessioned2017-04-06T01:02:12Z
dc.date.available2017-04-06T01:02:12Z
dc.identifierFEMS Microbiology Letters, v. 208, n. 2, p. 163-168, 2002.
dc.identifier0378-1097
dc.identifierhttp://hdl.handle.net/11449/66849
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66849
dc.identifier10.1016/S0378-1097(01)00473-6
dc.identifierWOS:000175152500002
dc.identifier2-s2.0-0037022952.pdf
dc.identifier2-s2.0-0037022952
dc.identifierhttp://dx.doi.org/10.1016/S0378-1097(01)00473-6
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888365
dc.descriptionTransposons are mobile genetic elements found within the genomes of various organisms including bacteria, fungi, plants and animals. Fragments of the transposon Tn1721 were found included in the genome of Xylella fastidiosa strain 9a5c. Regions from such fragments were PCR-amplified using specially designed primers (TNP1 and TNP2). In order to detect insertions of the Tn1721 element, both primers were used and one of them included a region of the transposon (TNP1) and the other one had the right repeat and part of the bacterial chromosome (TNP2). The PCR products obtained from strain 9a5c were used as a pattern for fragment size comparisons when DNA samples from other X. fastidiosa strains were used as template for the PCR assays. Differences were observed concerning the PCR products of such amplifications when some X. fastidiosa strains isolated from grapevine and plum were used. For the citrus-derived strains only the strains U187d and GP920b produced fragments with different sizes or weak band intensity. Such variations in the X. fastidiosa genome related to disrupted Tn1721 copies are probably due to the possibility of such a transposon element being still able to duplicate even after deletion events might have taken place and also because the bacterial strains in which the main differences were detected are derived from different host plants cultivated under different climate conditions from the one used as reference. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.languageeng
dc.relationFEMS Microbiology Letters
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPCR fragment
dc.subjectTransposon Tn1721
dc.subjectXylella fastidiosa
dc.subjectbacterial DNA
dc.subjectbacterial chromosome
dc.subjectbacterial genome
dc.subjectbacterial strain
dc.subjectbacterium isolation
dc.subjectcitrus fruit
dc.subjectcontrolled study
dc.subjectDNA template
dc.subjectgene amplification
dc.subjectgene deletion
dc.subjectgene duplication
dc.subjectgenetic variability
dc.subjectGram negative bacterium
dc.subjectnonhuman
dc.subjectplum
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjecttransposon
dc.subjectxylell fastidiosa
dc.subjectBase Sequence
dc.subjectCitrus
dc.subjectCoffee
dc.subjectDNA Transposable Elements
dc.subjectDNA, Bacterial
dc.subjectGammaproteobacteria
dc.subjectMolecular Sequence Data
dc.subjectPlant Diseases
dc.subjectPrunus
dc.subjectSequence Alignment
dc.subjectSpecies Specificity
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectFungi
dc.subjectPrunus domestica
dc.subjecttransposons
dc.subjectVitis
dc.titleTransposon Tn1721 distribution among strains of Xylella fastidiosa
dc.typeOtro


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