Calliandra surinamensis lectin (CasuL) does not impair the functionality of mice splenocytes, promoting cell signaling and cytokine production

dc.creatorProcópio, Thamara Figueiredo
dc.creatorSiqueira Patriota, Leydianne Leite de
dc.creatorSilva Barros, Bárbara Rafaela da
dc.creatorSouza Aguiar, Lethícia Maria de
dc.creatorLorena, Virgínia Maria Barros de
dc.creatorPaiva, Patrícia Maria Guedes
dc.creatorMelo, Cristiane Moutinho Lagos de
dc.creatorNapoleão, Thiago Henrique
dc.date2019-04-17T13:50:48Z
dc.date2019-04-17T13:50:48Z
dc.date2018
dc.date.accessioned2023-09-26T20:40:59Z
dc.date.available2023-09-26T20:40:59Z
dc.identifierPROCÓPIO, T. F. et al. Calliandra Surinamensis Lectin (CasuL) Does Not Impair the Functionality of Mice Splenocytes, Promoting Cell Signaling and Cytokine Production. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, v. 107, p. 650–655, nov. 2018.
dc.identifier1950-6007
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32552
dc.identifier10.1016/j.biopha.2018.08.043
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861931
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 446902 / 2014-4 ) para bolsas de pesquisa e bolsas de estudo (PMGP e THN), bem como para a Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco ( FACEPE; APQ-0108-2.08 / 14 ; APQ-0661-2.08 / 15 ) para apoio financeiro. A TFP gostaria de agradecer à FACEPE pela bolsa de pós-graduação ( IBPG-0088-2.08 / 14 ) e ao Núcleo de Plataformas Tecnológicas do Instituto Aggeu Magalhães, da Fundação Oswaldo Cruz (FIOCRUZ Pernambuco)
dc.descriptionCasuL is a lectin (carbohydrate-binding protein) isolated from the leaf pinnulae of Calliandra surinamensis that is toxic against cancer cells. In this study, the effects of CasuL on the activation of immune cells were evaluated in BALB/c mice splenocytes. Assays measuring the changes in cytosolic calcium concentration ([Ca2+]cyt), mitochondrial membrane potential (ΔΨm), and reactive oxygen species (ROS) levels associated with cell viability, proliferation, and cytokine and nitric oxide production were performed. The lectin (3.12-100 μg/mL) did not induce apoptosis or necrosis of splenocytes, and treatment for 48 h at 12.5 μg/mL stimulated cell proliferation. High cytosolic ROS levels were found in cells incubated with CasuL (12.5 μg/mL), but it did not affect [Ca2+]cyt, mitochondrial ROS, and ΔΨm levels. Furthermore, CasuL promoted high IL-2 and TNF-α production but did not affect nitric oxide release. In conclusion, CasuL was able to promote oxidative stress in mouse immune cells without inducing cell damage, and stimulated proliferation and cytokine production. These findings suggest the potential use of CasuL in future antitumoral and immunological targets.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectLeguminous
dc.subjectPlant lectin
dc.subjectOxidative stress
dc.subjectTumor necrosis factor
dc.subjectImmunomodulation
dc.subjectAnimais
dc.subjectMorte Celular / efeitos de drogas
dc.subjectProliferação Celular / efeitos de drogas
dc.subjectCitocinas / biossíntese
dc.subjectFabaceae / química
dc.subjectFêmea
dc.subjectLectinas / farmacologia
dc.subjectRatos, endogâmicos BALB C
dc.subjectCoelhos
dc.subjectTransdução de Sinal / efeitos de drogas
dc.subjectBaço / metabolismo
dc.titleCalliandra surinamensis lectin (CasuL) does not impair the functionality of mice splenocytes, promoting cell signaling and cytokine production
dc.typeArticle


Este ítem pertenece a la siguiente institución