Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

dc.creatorMalta, Fábio Correia
dc.creatorVarella, Rafael Brandão
dc.creatorGuimarães, Maria Angelica Arpon Marandino
dc.creatorMiagostovich, Marize Pereira
dc.creatorFumian, Tulio Machado
dc.date2021-01-15T13:54:39Z
dc.date2021-01-15T13:54:39Z
dc.date2020
dc.date.accessioned2023-09-26T20:23:03Z
dc.date.available2023-09-26T20:23:03Z
dc.identifierMALTA, Fábio Correia et al. Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections. Pathogens, v. 9, n. 645, p. 1-14, 2020.
dc.identifier2076-0817
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/45714
dc.identifier10.3390/pathogens9080645
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855683
dc.descriptionHuman bocavirus (HBoV) is an emerging virus and has been detected worldwide, especially in pediatric patients with respiratory and gastrointestinal infection. In this study, we describe HBoV prevalence, genotypes circulation andDNAshedding, in stool samples fromchildren up to two years of age in Brazil. During 2016 and 2017, 886 acute gastroenteritis (AGE) stool samples from ten Brazilian states were analyzed by TaqMan®-based qPCR, to detect and quantify HBoV. Positive samples were genotyped by sequencing the VP1/2 overlap region, followed by phylogenetic analysis and co-infections were accessed by screening other gastroenteric viruses. HBoV was detected in 12.4% (n = 110) of samples, with viral load ranging from 1.6 102 to 1.2 109 genome copies per gram of stool. From these, co-infections were found in 79.1%, and a statistically lower HBoV viral load was found compared to viral loads of rotavirus, norovirus and adenovirus in double infected patients (p < 0.05). No significant di erences were found between HBoV viral load in single or co-infections, age groups or genotypes. Phylogenetic analysis identified the circulation of HBoV-1 in 38%, HBoV-2 in 40% and HBoV-3 in 22%. Continuous HBoV monitoring is needed to clarify its role in diarrhea disease, especially in the absence of classic gastroenteric viruses.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectGastroenterite aguda
dc.subjectBocavírus humano
dc.subjectCo-infecções
dc.subjectCarga viral
dc.subjectGenotipagem
dc.subjectBrasil
dc.subjectAcute gastroenteritis
dc.subjectHuman bocavirus
dc.subjectCo-infections
dc.subjectViral load
dc.subjectGenotyping
dc.subjectBrazil
dc.titleHuman Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
dc.typeArticle


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