dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFeldman, Mark
dc.creatorVu Dang La,
dc.creatorLombardo Bedran, Telma Blanca
dc.creatorPalomari Spolidorio, Denise Madalena
dc.creatorGrenier, Daniel
dc.date2014-05-20T15:32:06Z
dc.date2016-10-25T18:08:13Z
dc.date2014-05-20T15:32:06Z
dc.date2016-10-25T18:08:13Z
dc.date2011-12-01
dc.date.accessioned2017-04-06T00:25:56Z
dc.date.available2017-04-06T00:25:56Z
dc.identifierMicrobes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 14-15, p. 1261-1269, 2011.
dc.identifier1286-4579
dc.identifierhttp://hdl.handle.net/11449/41094
dc.identifierhttp://acervodigital.unesp.br/handle/11449/41094
dc.identifier10.1016/j.micinf.2011.07.009
dc.identifierWOS:000298127800015
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2011.07.009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/883818
dc.descriptionExtracellular matrix metalloproteinase inducer (EMMPRIN) or CD 147 is a transmembrane glycoprotein expressed by various cell types, including oral epithelial cells. Recent studies have brought evidence that EMMPRIN plays a role in periodontitis. In the present study, we investigated the effect of Porphyromonas gingivalis, a major pathogen in chronic periodontitis, on the shedding of membrane-anchored EMMPRIN and on the expression of the EMMPRIN gene by oral epithelial cells. A potential contribution of shed EMMPRIN to the inflammatory process of periodontitis was analyzed by evaluating the effect of recombinant EMMPRIN on cytokine and matrix metalloproteinase (MMP) secretion by human gingival fibroblasts. ELISA and immunofluorescence analyses revealed that P. gingivalis mediated the shedding of epithelial cell-surface EMMPRIN in a dose- and time-dependent manner. Cysteine proteinase (gingipain)-deficient P. gingivalis mutants were used to demonstrate that both Arg- and Lys-gingipain activities are involved in EMMPRIN shedding. Real-time PCR showed that P. gingivalis had no significant effect on the expression of the EMMPRIN gene in epithelial cells. Recombinant EMMPRIN induced the secretion of IL-6 and MMP-3 by gingival fibroblasts, a phenomenon that appears to involve mitogen activated protein kinases. The present study brought to light a new mechanism by which P. gingivalis can promote the inflammatory response during periodontitis. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCytokine
dc.subjectEMMPRIN
dc.subjectEpithelial cell
dc.subjectFibroblast
dc.subjectPeriodontitis
dc.subjectPorphyromonas gingivalis
dc.titlePorphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer (EMMPRIN) by oral epithelial cells: a potential role in inflammatory periodontal disease
dc.typeOtro


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