Trabajo de grado - Maestría
Effect of lipids during Malassezia spp. infection in keratinocytes
Fecha
2022-12-14Registro en:
instname:Universidad de los Andes
reponame:Repositorio Institucional Séneca
Autor
Mora Restrepo, Felipe
Institución
Resumen
The epidermis is the first protective barrier against pathogenic agents. It hosts a complex of beneficial microorganisms for the skin, such as lipid-dependent yeasts of the Malassezia genus. In the epidermis, this yeast interacts with keratinocytes while follicles that contain the sebaceous glands with a variety of lipids such as squalene, triglycerides, free fatty acids, and others, turning the skin into a suitable place for these yeasts to establish their ecological niche. Here, we evaluated the effect for the addition of representative fatty acids to the skin, stearic acid, oleic acid, and squalene, in the interaction of M. furfur, M. restricta, and M. sympodialis with the HEKa human keratinocyte cell line. Adding fatty acids changes the interaction process, decreased cell viability, and cause a cytotoxic effect on keratinocytes. M. furfur, together with M. sympodialis, showed a negative impact against keratinocytes and is increased by the presence of fatty acids. Otherwise, the presence of lipids alone is not enough to promote the growth of M. sympodialis and M. restricta, showing that the conditions for its establishment in the skin are complex and specific processes could be species specific.