Dissertação
Avaliação da atividade das colinesterases, adenosina desaminase e biomarcadores de inflamação em pacientes com esclerose múltipla
Fecha
2013-08-19Autor
Polachini, Carla Roberta Nunes
Institución
Resumen
Multiple sclerosis (MS) is an autoimmune disease complex,
since several pathophysiological
processes, including inf lammation, demyelination, axonal damage and repair mechanisms
are involved in its eme rgence. It attacks the central nervous system (CNS) and may affect
both brain and bone s . Research es ha ve shown that changes in the immune response and
the presence of pro inflammatory events may lead to the emergence of patholog ies and
relapses in the MS. In this context, it becomes important to study cholinesterase enzymes
such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as well as the
inflammatory biomarkers and nucleotides and nucleosides of adenine and adenos ine
deaminase (ADA) because all these parameters are involved in regulating the immune
system and pr o and/or anti inflammatory processes. Thus, the objective of this study was to
verify the AChE activity in lymphocytes and whole blood as well as the activ ity of BChE and
ADA enzymes and the levels of IL 1, IL 6, IL 10, IFN γ, TNF α and C reactive protein (CRP)
in serum. Additionally, we evaluated the levels of ATP, ADP, AMP, adenosine, inosine,
hypoxanthine and uric acid in heal thy subjects and in the seru m of patients with the
rel apsing remitting form of MS (RR M S Results showed a significant increase in the AChE
activity in lymphocytes a s well as whole blood and serum BChE in patients compared with
heal thy subjects (P <0.05). Also, we observed an increas e in serum levels of IL 1, IL 6, IFN
γ, TNF α and C reactive protein (CRP) and a decrea se in IL 10 in patients with RR M S when
compared to the control group. Another imp ortant finding was the increased ADA activi ty in
serum in patients with RR M S compared to the control group. Furthermore , results of the
nucleotide analysis in serum showed that the levels of ADP, AMP and adenosine wer e
increased in carries of RR M S , whereas ATP levels were decreased in this group. The results
described here suggest that the ch olinesterase activity, biomarkers and purinergic
inflammatory are altered in patients with RR M S , suggesting thus an exacerbated
inflammatory response. These findings may be useful to extend the therapeutic window for
the treatment and monitoring of this di sease.