Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCardoso, Tereza C.
dc.creatorFerrari, Heitor F.
dc.creatorBregano, Livia C.
dc.creatorSilva-Frade, Camila
dc.creatorRosa, Ana Carolina G.
dc.creatorAndrade, Alexandre Lima de
dc.date2013-09-30T19:14:53Z
dc.date2014-05-20T13:42:39Z
dc.date2016-10-25T16:57:24Z
dc.date2013-09-30T19:14:53Z
dc.date2014-05-20T13:42:39Z
dc.date2016-10-25T16:57:24Z
dc.date2010-12-01
dc.date.accessioned2017-04-05T20:44:55Z
dc.date.available2017-04-05T20:44:55Z
dc.identifierMolecular and Cellular Probes. London: Academic Press Ltd- Elsevier B.V. Ltd, v. 24, n. 6, p. 415-417, 2010.
dc.identifier0890-8508
dc.identifierhttp://hdl.handle.net/11449/14840
dc.identifierhttp://acervodigital.unesp.br/handle/11449/14840
dc.identifier10.1016/j.mcp.2010.08.003
dc.identifierWOS:000283970200015
dc.identifierhttp://dx.doi.org/10.1016/j.mcp.2010.08.003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/861929
dc.descriptionA sensitive reverse-transcription loop-mediated isothermal amplification (RI-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection The reaction is performed in one step in a single tube at 65 degrees C for 45 min with hydroxynaphthol blue (HNB) dye added prior to amplification The detection limit of the RI-LAMP assay was approximately 10(2) EID50/50 mu l TCoV genome and no cross-reaction with other avian viruses was observed The assay was evaluated further in tissue suspensions prepared from the ileum and ileum-caecal junctions of infected turkey embryos 100% of these samples were positive in the RI-LAMP assay All individual feces samples collected in the field were considered positive by both conventional RT-PCR and RI-LAMP In conclusion RI-LAMP with HNB dye was shown to be a sensitive simple assay for the rapid diagnosis of TCoV infection either directly from feces or in association with virus isolation methods (C) 2010 Elsevier Ltd All rights reserved
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherAcademic Press Ltd Elsevier B.V. Ltd
dc.relationMolecular and Cellular Probes
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTurkey coronavirus
dc.subjectHydroxynaphthol blue
dc.subjectLAMP assay
dc.subjectSpike gene
dc.titleVisual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye
dc.typeOtro


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