Artigo
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation
Fecha
2012-01-01Registro en:
Journal of Biomedicine and Biotechnology. New York: Hindawi Publishing Corporation, 10 p., 2012.
1110-7243
WOS000304937000001.pdf
10.1155/2012/649353
WOS:000304937000001
Autor
Janz, Felipe de Lara
Debes, Adriana de Aguiar
Cavaglieri, Rita de Cassia
Duarte, Sergio Aloisio
Romao, Carolina Martinez
Moron, Antonio Fernandes [UNIFESP]
Zugaib, Marcelo [UNIFESP]
Bydlowski, Sergio Paulo
Institución
Resumen
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. in this concern, dimethyl sulfoxide (Me2SO) showed the best results. the freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios.