info:eu-repo/semantics/article
ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
Autor
Nicolas, Juan Carlos
Grumelli, Yanina Alejandra
Institución
Resumen
Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples. Fil: Nicolas, Juan Carlos. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Grumelli, Yanina Alejandra. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina