info:eu-repo/semantics/article
Decreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis
Registro en:
Marcos CM, Silva Jde F, Oliveira HC, Assato PA, Singulani Jde L, Lopez AM, Tamayo DP, Hernandez-Ruiz O, McEwen JG, Mendes-Giannini MJ, Fusco-Almeida AM. Decreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis. Virulence. 2016;7(2):72-84. doi: 10.1080/21505594.2015.1122166.
2150-5594
10.1080/21505594.2015.1122166.
2150-5608
Autor
Marcos, Caroline María
Da Silva, Julhiany de Fátima
De Oliveira, Haroldo Cesar
Akemi Assato, Patrícia
De Lacorte Singulani, Junya
López García, Ángela María
Tamayo Ossa, Diana Patricia
Hernández Ruiz, Orville
McEwen Ochoa, Juan Guillermo
Soares Mendes Giannini, Maria José
Fusco Almeida, Ana Marisa
Institución
Resumen
ABSTRACT : The interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 protein. The 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. Here, we investigated the relevance of the 14-3-3 protein to the virulence of P. brasiliensis. Using antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated a 14-3-3-silenced strain (expression reduced by~55%). This strain allowed us to investigate the interaction between 14-3-3 and the host and to correlate the functions of P. brasiliensis 14-3-3 with cellular features, such as morphological characteristics and virulence, that are important for pathogenesis. COL0000962 COL0126131