info:eu-repo/semantics/article
Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture
Registro en:
Ospina, S. P., Ramírez, D. A., Escobar, D. M., Ossa, C. P., Rojas, D. F., Zapata, P. & Atehortúa, L. (2014).Comparison of extraction methods of chitin from ganoderma lucidum mushroom obtained in submerged culture. BioMed Research International, 2014, 1-6. DOI: 10.1155/2014/169071
2314-6133
10.1155/2014/169071
2314-6141
Autor
Ramírez Cadavid, David Alexander
Escobar Sierra, Diana Marcela
Ossa Orozco, Claudia Patricia
Rojas Vahos, Diego Fernando
Zapata Ocampo, Paola Andrea
Atehortúa Garcés, Lucía
Institución
Resumen
ABSTRACT: The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under
biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases:
pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium
permanganate and oxalic acid. The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g−1 (milligrams
of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively. Obtained chitin was characterized by X-Ray Diffraction
(XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA). The results showed that Ganoderma
lucidum chitin has similar characteristic of chitin from different fonts. The advantage of the biotechnological processes and the fact
that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.