Tesis Magíster
Efecto del pre-condicionamiento de células estromales multipotentes equinas obtenidas de médula ósea, sobre su capacidad de inhibir la proliferación de linfocitos
Autor
Caffi-Pizarro, Valeria Camila
Institución
Resumen
Mesenchymal stem cells (MSCs) are more commonly used every day in both human clinical trials and in veterinary medicine for the treatment of inflammatory diseases. MSCs modulate inflammation through the secretion of mediators. However, despite widespread clinical use, knowledge regarding the effector mechanisms of equine MSCs and consequently their effectiveness in the treatment of diseases is still unknown. The objective of this study was to evaluate the effect of preconditioning of equine MSCs derived from bone marrow, with different pro-inflammatory molecules, on the capacity of these to inhibit the proliferation of lymphocytes. It could be determined that the MSCs were able to significantly inhibit the proliferation of lymphocytes, and that in order to achieve, the production of prostaglandin E2 (PGE2) and the activity of indoleamine 2,3-dioxygenase (IDO) were necessary. On the other hand, the pre-conditioning of the MSCs with TNF-α, IFN-γ or their combination significantly increased the expression of important immunomodulatory molecules such as IL-6, IDO, iNOS and COX- 2. However, this did not correlate with an increase in the ability of MSCs to inhibit lymphocyte proliferation. PFCHA-Becas PFCHA-Becas