Principales marcadores moleculares utilizados para la identificación de Babesia bovis y Babesia bigemina

dc.creatorRíos T, Sandra
dc.creatorRíos O, Leonardo Ríos O
dc.date2011-05-10
dc.date.accessioned2022-12-15T14:42:32Z
dc.date.available2022-12-15T14:42:32Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/1006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353158
dc.descriptionThis paper describes the principal molecular markers used to identify B. bovis and B. bigemina reported in the scientific literature. A systematic review was designed based on the application of the PICO methodologic modified strategy to define the nucleotide sequences detected in different geographical locations and their diagnostic utility. An advanced search was made in data bases ScienceDirect, SpringerLink and PubMed. Using the terms “Babesia bovis” and “DNA” and “Babesia bigemina” and “DNA”. A total of 68 original articles were selected after the information was filtered. Both included and excluded articles were registered in tables, where its position of their status, within the study, was represented. The 68 articles were evaluated using a previously defined criteria of inclusion or exclusion. A total of 21 articles met the inclusion criteria and were included in this study. It was described the usefulness of molecular markers referenced in the scientific literature from 1995 to 2010: small-subunit ribosomal rna, cytochrome b gene, msa-1 and msa-2c gene, bv gene, elongation factor –1 alpha (ef-1α), beta-tubulin gene, sbp 1-2-3, and rap genes, its diagnostic application and its use in different geographical locations. Molecular markers used for detection of bovine babesia vary by geographic region, degree of genetic conservation and results of previous studies that conclude their diagnostic utility.en-US
dc.description Este artículo describe los principales marcadores moleculares utilizados para la identificación de B. bovis y B. bigemina reportados en la literatura científica. Para ello se diseñó una revisión sistemática a partir de la aplicación de la estrategia metodológica PICO modificada con el objetivo de definir las secuencias nucleotídicas detectadas en los diferentes sitios geográficos y su utilidad diagnóstica. Se realizó una búsqueda avanzada con los términos “Babesia bovis” y “DNA” y “Babesia bigemina” y “DNA” en las bases de datos ScienceDirect, SpringerLink y PubMed que después de ser filtradas permitieron obtener un resultado total de 68 artículos originales. Tanto los artículos incluidos como los excluidos fueron almacenados en tablas, en las cuales se presenta la justificación de su condición dentro del estudio. A los 68 artículos seleccionados se les aplicó una evaluación con criterios de inclusión y exclusión previamente definidos, de este modo, 21 artículos originales cumplieron con los criterios de inclusión y se incluyeron en el estudio. Se describe la utilidad de los marcadores moleculares referenciados en la literatura científica desde 1995 hasta el 2010: la subunidad pequeña RNAr, el gen citocromo b, gen msa-1 and msa-2c, el gen Bv, el factor de elongación alfa (EF-1α), el gen de la beta-Tubulina, SBP 1-2-3, y los RAP; su aplicación diagnóstica y su utilización en los diferentes sitios geográficos. Los marcadores moleculares utilizados para la detección de las babesias bovinas varían dependiendo de la región geográfica, grado de conservación genética y resultados de estudios previos que concluyen su utilidad diagnóstica.es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1006/1237
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dc.sourceJournal MVZ Cordoba; Vol. 16 No. 2 (2011): Revista MVZ Córdoba Volumen 16(2) Mayo-Agosto 2011; 2470-2483en-US
dc.sourceRevista MVZ Córdoba; Vol. 16 Núm. 2 (2011): Revista MVZ Córdoba Volumen 16(2) Mayo-Agosto 2011; 2470-2483es-ES
dc.source1909-0544
dc.source0122-0268
dc.subjectCytochrome ben-US
dc.subjectDNAen-US
dc.subjectgenetic markersen-US
dc.subjectnucleotide sequencesen-US
dc.subjecttubulinen-US
dc.subjectCitocromo bes-ES
dc.subjectDNAes-ES
dc.subjectmarcadores genéticoses-ES
dc.subjectsecuencias nucleotídicases-ES
dc.subjecttubulinaes-ES
dc.titlePrincipal molecular markers used to identify Babesia bovis and Babesia bigeminaen-US
dc.titlePrincipales marcadores moleculares utilizados para la identificación de Babesia bovis y Babesia bigeminaes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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