Uterine luminal fluid protein content in viable and non-viable conceptuses in sheep
Contenido de proteínas del fluido luminal uterino en conceptus ovinos viables y no viables
| dc.creator | Rodríguez Márquez, José | |
| dc.creator | Hernández, Aureliano | |
| dc.creator | Vera, Víctor | |
| dc.creator | Morales, Roneisa | |
| dc.date | 2012-01-13T10:08:08Z | |
| dc.date | 2012-01-13T10:08:08Z | |
| dc.date | 2012-01-13T10:08:08Z | |
| dc.date.accessioned | 2022-11-17T00:10:09Z | |
| dc.date.available | 2022-11-17T00:10:09Z | |
| dc.identifier | 0798-2259 | |
| dc.identifier | http://www.saber.ula.ve/handle/123456789/34377 | |
| dc.identifier | 199102ZU46 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5208719 | |
| dc.description | During implantation, embryo survival depends on the availability of uterine and/or trophoblastic secreted molecules, including proteins. In order to determine possible differences in the protein profile of the uterine luminal fluid (ULF) adjacent to the embryonic site of viable and non-viable conceptuses at 20, 28 and 35 days of gestation, two-dimensional (2D) PAGE electrophoresis was carried out. The ULF adjacent to viable conceptuses, had greater amounts of proteins as compared to correspondent sites of nonviable conceptuses. In all the studied gestational period there were several proteins in the ULF near viable embryos, which were absent in the corresponding areas of the non viable ones. Two of these proteins were identified. One corresponds to transferrin, which can be produced either by the conceptus or the mother. It is important for proliferation, differentiation, and cellular function, since it is an iron transporting and binding molecule. Its absence in the ULF near non viable embryos might be interpreted as a lack of production by the conceptus. Likewise, transferrin appears to be involved in the formation of blood vessels, which then could account for the absence of those structures in the membranes of non viable conceptuses. The other protein corresponds to aldose reductase, which is responsible for sorbitol and fructose synthesis, although its metabolic role during implantation is still unknown. In conclusion, some proteins present near viable embryos are absent in non viable conceptuses at days 20; 28 and 35 of gestation which could be indicators to determine, at least partially, embryonic survival during the studied period. | |
| dc.description | Durante la implantación, la supervivencia del embrión depende de la habilidad del útero y/o trofoblasto para secretar moléculas, incluyendo proteínas. Para determinar las diferencias posibles en el perfil proteico del fluido luminal uterino (FLU) adyacente al embrión de conceptus viables y no viables a los 20, 28 y 35 días de gestación, se realizó electroforesis bidimensional (2DPAGE- SDS). El FLU adyacente a conceptus viables, tuvo mayor cantidad de proteínas al compararlo al correspondiente de conceptus no viables. En todos los periodos de gestación estudiados hubo varias proteínas en el FLU cerca de embriones viables, las cuales estuvieron ausentes en la correspondiente área de los embriones no viables. Dos de estas proteínas fueron identificadas. Una de ellas corresponde a la transferrina, la cual puede ser producida, tanto por la madre como por el conceptus, y es importante para la proliferación, diferenciación y función celular, debido a su capacidad de unir y transportar hierro. Su ausencia cerca de los embriones no viables es interpretada como una falla en la producción por el conceptus. También, la transferrina parece estar involucrada en la formación de vasos sanguíneos, la cual podría ser responsable de la ausencia de esta estructura en las membranas de conceptus no viables. La otra proteína encontrada correspondió a la aldosa reductasa, la cual es responsable de la síntesis de fructosa y sorbitol, aunque el papel de este metabolito durante al implantación es desconocido. En conclusión, algunas proteínas presentes cerca de embriones viables estuvieron ausentes en conceptus no viables a los días 20; 28 y 35 de gestación, lo cual puede ser un indicador para determinar, al menos, parcialmente, la supervivencia embrionaria durante el periodo estudiado. | |
| dc.description | 539 - 547 | |
| dc.description | jose.rodriguez@fcv.luz.edu.ve | |
| dc.description | Bimestral | |
| dc.format | application/pdf | |
| dc.language | es | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/3.0/ve/ | |
| dc.subject | Proteins | |
| dc.subject | Uterine fluid | |
| dc.subject | Embryonic mortality | |
| dc.subject | Implantation | |
| dc.subject | Sheep | |
| dc.subject | Universidad del Zulia (LUZ) | |
| dc.subject | Universidad de Los Andes (ULA) | |
| dc.subject | Proteínas | |
| dc.subject | Implantación | |
| dc.subject | Revista Científica | |
| dc.subject | Revista Científica: Medicina Veterinaria | |
| dc.subject | Medio Ambiente | |
| dc.subject | Revistas | |
| dc.title | Uterine luminal fluid protein content in viable and non-viable conceptuses in sheep | |
| dc.title | Contenido de proteínas del fluido luminal uterino en conceptus ovinos viables y no viables | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | Texto |