Article
The ATP levels in kidneys and blood are mainly decreased by acute ingestion of tullidora (Karwinskia humboldtiana)
Fecha
2005Autor
Jaramillo-Juarez, F.
Rodriguez-Vazquez, M.L.
Munoz-Martinez, J.
Quezada-Tristan, T.
Posadas Del Rio, F.A.
Llamas-Viramontes, J.
Ortiz, G.G.
Feria-Velasco, A.
Reyes, J.L.
Institución
Resumen
Our previous acute toxicity studies with Karwinskia humboldtiana (Kh) in rats showed renal hemodynamic changes with a marked increase in the fractional excretion of sodium and morphological damage. To analyse the effects of Kh or 'tullidora' on energetic metabolism, a single dose of an oral preparation from the seed fruits was given to Wistar rats (1.25 g/kg). In tullidora-treated rats there was 8% mortality. ATP concentrations in renal tissue decreased significantly (control: 53.85±3.34, tullidora 38.28±5.31 μmol/g fresh tissue, P<0.05). Total blood (54.8±0.96, tullidora: 40.2±1.55 μmol/dL, P<0.01) and haemoglobin-ATP concentrations (3.69±0.12, tullidora: 2.56±0.11 μmol/g, P<0.01) were also significantly diminished. Moreover, the total protein in renal cortex from tullidora-treated rats decreased as compared to control group (control: 71.43±2.88, tullidora: 55.20±4.06 mg/g fresh tissue, P<0.05). In contrast, Na+-K+-ATPase activity in tullidora-treated animals was not different from control rats. These findings might partially explain the acute effects and mortality observed in the Kh treated rats. © 2005 Elsevier Ltd. All rights reserved.