info:eu-repo/semantics/article
The phthalate DEHP modulates the estrogen receptors α and β increasing lactotroph cell population in female pituitary glands
Fecha
2020-11Registro en:
Pérez, Pablo Aníbal; Toledo, Jonathan; Sosa, Liliana del Valle; Peinetti, Nahuel; Torres, Alicia Ines; et al.; The phthalate DEHP modulates the estrogen receptors α and β increasing lactotroph cell population in female pituitary glands; Pergamon-Elsevier Science Ltd; Chemosphere; 258; 11-2020; 1-27
0045-6535
CONICET Digital
CONICET
Autor
Pérez, Pablo Aníbal
Toledo, Jonathan
Sosa, Liliana del Valle
Peinetti, Nahuel
Torres, Alicia Ines
de Paul, Ana Lucia
Gutiérrez, Silvina
Resumen
Humans are exposed to numerous endocrine disruptors on a daily basis, which may interfere with endogenous estrogens, with Di-(2-ethylhexyl) phthalate (DEHP) being one of the most employed. The anterior pituitary gland is a target of 17β-estradiol (E2) through the specific estrogen receptors (ERs) α and β, whose expression levels fluctuate in the gland under different contexts, and the ERα/β index is responsible for the final E2 effect. The aim of the present study was to evaluate in vivo and in vitro the DEHP effects on ERα and β expression in the pituitary cell population, and also its impact on lactotroph and somatotroph cell growth. Our results revealed that perinatal exposure to DEHP altered the ERα and β expression pattern in pituitary glands from prepubertal and adult female rats and increased the percentage of lactotroph cells in adulthood. In the in vitro system, DEHP down-regulated ERα and β expression, and as a result increased the ERα/β ratio and decreased the percentages of lactotrophs and somatotrophs expressing ERα and β. In addition, DEHP increased the S + G2M phases, Ki67 index and cyclin D1 in vitro, leading to a rise in the lactotroph and somatotroph cell populations. These results showed that DEHP modified the pituitary ERα and β expression in lactotrophs and somatotrophs from female rats and had an impact on the pituitary cell growth. These changes in ER expression may be a mechanism underlying DEHP exposure in the pituitary gland, leading to cell growth deregulation.