dc.creator | Careaga Quiroga, Valeria Pilar | |
dc.creator | Bueno, Carlos Alberto | |
dc.creator | Muniain, Claudia Cristina | |
dc.creator | Alche, Laura Edith | |
dc.creator | Maier, Marta Silvia | |
dc.date.accessioned | 2020-02-12T22:52:52Z | |
dc.date.accessioned | 2022-10-15T12:51:16Z | |
dc.date.available | 2020-02-12T22:52:52Z | |
dc.date.available | 2022-10-15T12:51:16Z | |
dc.date.created | 2020-02-12T22:52:52Z | |
dc.date.issued | 2009-12 | |
dc.identifier | Careaga Quiroga, Valeria Pilar; Bueno, Carlos Alberto; Muniain, Claudia Cristina; Alche, Laura Edith; Maier, Marta Silvia; Antiproliferative, cytotoxic and hemolytic activities of a triterpene glycoside from Psolus patagonicus and its desulfated analog; Karger; Chemotherapy; 55; 1; 12-2009; 60-68 | |
dc.identifier | 0009-3157 | |
dc.identifier | http://hdl.handle.net/11336/97368 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4388121 | |
dc.description.abstract | Background: The major triterpene glycoside of the sea cucumber Psolus patagonicus and its desulfated analog were tested for their antiproliferative, cytotoxic and hemolytic activities, and their effect on NF-κB activation. Methods: The antiproliferative action of glycosides 1 and 2 were determined on 3 tumor cell lines. Their effect on the activation of NF-κB was evaluated by indirect immunofluorescence assay staining and the concomitant IκBα degradation was studied by Western blot. Results: Both compounds were able to suppress the growth of 3 tumor cell lines (Hep3B, MDA-MB231 and A549) and induced the activation of NF-κB, a key player linking chronic inflammation and cancer, concomitant with IκBα degradation in the A549 tumor cell line. Compounds 1 and 2 showed hemolytic activity with half maximal inhibitory concentration (IC50) values around 80 μM. Conclusions: Both glycosides showed low cytotoxic activity in A549 tumor cells in comparison with sea cucumber triterpene glycosides containing a linear tetrasaccharide chain. This could be a result of the uncommon presence of two 12α- and 17α-hydroxyl groups and a Δ7 double bond in the aglycone moiety. This aglycone functionalization may be related to their low membranolytic activity. Although glycosides 1 and 2 exert an antiproliferative effect, their mechanisms of action do not involve inhibition of NF-κB. Recently, it has been shown that diverse and new mechanisms of action are responsible for the antitumor and cytotoxic activities of marine compounds. Therefore, more extensive studies are needed to establish a mechanism of action and to deduce a clear structure-activity relationship of sea cucumber triterpene glycosides. | |
dc.language | eng | |
dc.publisher | Karger | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000180340 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/180340 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | ANTIPROLIFERATION | |
dc.subject | CYTOTOXICITY | |
dc.subject | NUCLEAR FACTOR-KAPPA B | |
dc.subject | PSOLUS PATAGONICUS | |
dc.subject | SEA CUCUMBER | |
dc.subject | TRITERPENE GLYCOSIDES | |
dc.title | Antiproliferative, cytotoxic and hemolytic activities of a triterpene glycoside from Psolus patagonicus and its desulfated analog | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |