info:eu-repo/semantics/article
Binding of the highly toxic tetracycline derivative, anhydrotetracycline, to bovine serum albumin
Fecha
2011-08Registro en:
Burgos, Martha Ines; Fernández, Ricardo Ariel; Celej, Maria Soledad; Rossi, Laura Isabel; Fidelio, Gerardo Daniel; et al.; Binding of the highly toxic tetracycline derivative, anhydrotetracycline, to bovine serum albumin; Pharmaceutical Soc Japan; Biol. Pharm. Bull.; 34; 8; 8-2011; 1301-1306
0918-6158
1347-5215
CONICET Digital
CONICET
Autor
Burgos, Martha Ines
Fernández, Ricardo Ariel
Celej, Maria Soledad
Rossi, Laura Isabel
Fidelio, Gerardo Daniel
Dassie, Sergio Alberto
Resumen
Tetracycline (TC) derivatives are extensively used as antibiotics in human and animal medicine and, very recently, they have been screened as anti-amyloidogenic drugs. Anhydrotetracycline (AHTC) is one of the major degradation products of TC that has been linked to several side effects of the drug. We evaluated the interaction of AHTC with bovine serum albumin (BSA), one of the main carriers of amphiphilic molecules in blood, using three complementary analytical methods: fluorescence spectroscopy, isothermal titration calorimetry and differential scanning calorimetry. AHTC bound to BSA with an association constant in the order of 105M1. Drug binding was enthalpically and entropically driven and seemed to involve hydrophobic interactions. AHTC fluorescence enhancement and hypsochromic shifts observed upon binding suggested a low-polarity location excluded from water for the bound drug. Our data are useful for evaluating the biodisponibility of the pharmacophore and the dynamic distribution of the toxic derivative.