info:eu-repo/semantics/article
Lipofection of early passages of cell cultures derived from murine adenocarcinomas: In vitro and ex vivo testing of the thymidine kinase/ganciclovir system
Fecha
2002-12Registro en:
Karara, Armando Luis; Bumaschny, Viviana Florencia; Fiszman, Gabriel Leon; Casais, Cecilia C.; Glikin, Gerardo Claudio; et al.; Lipofection of early passages of cell cultures derived from murine adenocarcinomas: In vitro and ex vivo testing of the thymidine kinase/ganciclovir system; Nature Publishing Group; Cancer Gene Therapy; 9; 1; 12-2002; 96-99
0929-1903
CONICET Digital
CONICET
Autor
Karara, Armando Luis
Bumaschny, Viviana Florencia
Fiszman, Gabriel Leon
Casais, Cecilia C.
Glikin, Gerardo Claudio
Finocchiaro, Liliana Maria Elena
Resumen
Early passages of cultured cells derived from four spontaneous Balb/c murine adenocarcinomas were used to explore the feasibility of a nonviral HSVtk-based suicide gene therapy system. After lipofection with pCMVtk, the transiently HSVtk expressing P07 (lung), M3, M05, and M38 (mammary gland) cells were, respectively, about 130-, 30-, 120-, and 170-fold more sensitive to ganciclovir (GCV) in vitro than their respective controls. Eighty percent of Balb/c mice subcutaneously inoculated with ex vivo pCMVtk-lipofected P07 cells, followed by intraperitoneal GCV injection for 7 days, displayed a complete inhibition of tumor growth for over 70 days. Control animals started to display tumors 13 days after inoculation. We present evidence showing that early passages of cultured tumor cells can efficiently express lipofected genes and that they are sensitive to the lipoplex-mediated HSVtk/GCV system.