info:eu-repo/semantics/article
M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry
Fecha
2018-10Registro en:
Chauhan, Arun; Sun, Yuyang; Sukumaran, Pramod; Quenum Zangbede, Fredice O.; Jondle, Christopher N.; et al.; M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry; Elsevier Inc.; iScience; 8; 10-2018; 85-102
2589-0042
CONICET Digital
CONICET
Autor
Chauhan, Arun
Sun, Yuyang
Sukumaran, Pramod
Quenum Zangbede, Fredice O.
Jondle, Christopher N.
Sharma, Atul
Evans, Dustin L.
Chauhan, Pooja
Szlabick, Randolph E.
Aaland, Mary O.
Birnbaumer, Lutz
Sharma, Jyotika
Singh, Brij B.
Mishra, Bibhuti B.
Resumen
Macrophage plasticity is essential for innate immunity, but in-depth signaling mechanism(s) regulating their functional phenotypes are ill-defined. Here we report that interferon (IFN) g priming of naive macrophages induces store-mediated Ca2+ entry and inhibition of Ca2+ entry impairs polarization to M1 inflammatory phenotype. In vitro and in vivo functional analyses revealed ORAI1 to be a primary contributor to basal Ca2+ influx in macrophages, whereas IFNg-induced Ca2+ influx was mediated by TRPC1. Deficiency of TRPC1 displayed abrogated IFNg-induced M1 inflammatory mediators in macrophages. In a preclinical model of peritonitis by Klebsiella pneumoniae infection, macrophages showed increased Ca2+ influx, which was TRPC1 dependent. Macrophages from infected TRPC1 / mice showed inhibited expression of M1-associated signature molecules. Furthermore, in human patients with systemic inflammatory response syndrome, the level of TRPC1 expression in circulating macrophages directly correlated with M1 inflammatory mediators. Overall, TRPC1-mediated Ca2+ influx is essential for the induction/shaping of macrophage polarization to M1 inflammatory phenotype.