info:eu-repo/semantics/article
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
Fecha
2019-11Registro en:
Farace, Pablo Daniel; Morsella, Claudia G.; Cravero, Silvio Lorenzo Pedro; Sioya, Bernardo; Amadio, Ariel Fernando; et al.; L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus; PeerJ; PeerJ; 2019; 11; 11-2019; 1-21
2167-8359
CONICET Digital
CONICET
Autor
Farace, Pablo Daniel
Morsella, Claudia G.
Cravero, Silvio Lorenzo Pedro
Sioya, Bernardo
Amadio, Ariel Fernando
Paolicchi, Fernando Alberto
Gioffré, Andrea Karina
Resumen
Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogen sulfide production, for example, is a trait exclusive to C. fetus fetus and C. fetus venerealis biovar intermedius. This gas that can be biochemically tested can be produced from L-cysteine (L-Cys). Herein, we report a novel multiplex-PCR to differentiate C. fetus based on the evaluation of a deletion of an ATP-binding cassette-type L-Cys transporter that could be involved in hydrogen sulfide production, as previously described. A wet lab approach combined with an in silico whole genome data analysis showed complete agreement between this L-Cys transporter-PCR and the hydrogen sulfide production biochemical test. This multiplex-PCR may complement the tests currently employed for the differential diagnosis of C. fetus.