info:eu-repo/semantics/article
Analysis of single and multiple mutants constructs for human cytomegalovirus UL97 gene and its relationship with the phosphorylation activity of pUL97 kinase
Fecha
2009-11Registro en:
Sanchez Puch, Silvia; Mathet, Veronica Lidia; Porta, Margarita; Cuestas, María Luján; Oubiña, Jose Raul; et al.; Analysis of single and multiple mutants constructs for human cytomegalovirus UL97 gene and its relationship with the phosphorylation activity of pUL97 kinase; Elsevier; Antiviral Research; 84; 2; 11-2009; 194-198
0166-3542
CONICET Digital
CONICET
Autor
Sanchez Puch, Silvia
Mathet, Veronica Lidia
Porta, Margarita
Cuestas, María Luján
Oubiña, Jose Raul
Videla, Cristina Mónica
Salomon, Horacio Eduardo
Resumen
In this study we determined that the double mutant M460V/D605E in the UL97 gene of an HCMV isolate from an immunocompromised patient (MMT isolate) is related to resistance to ganciclovir (GCV) therapy. Our results suggest that the aspartic acid-to-glutamic acid substitution at codon 605 may be associated with a natural polymorphism of the UL97 gene, and not with positive selection pressure exerted by the antiviral drug. We also determined that GCV resistance due to the M460V mutation in the HCMV UL97 gene is not offset by a second mutation (D605E) at codon 605. Furthermore, we showed that when the two mutations related to GCV resistance were simultaneously detected in the same HCMV construct, virus-drug resistance might be enhanced in comparison to that of the single mutants studied separately. To our knowledge for the first time, seven of 12 amino acid changes (F102L, D118V, M330T, T400A, R507P and C511R and I533V) in the UL97 gene of an isolate are herein reported.