info:eu-repo/semantics/article
The role of PDLIM1, a PDZ-LIM domain protein, at the ribbon synapses in the chicken retina
Fecha
2020-07Registro en:
Rios, Hugo; Paganelli, Alejandra Raquel; Fosser, Nicolás Sebastián; The role of PDLIM1, a PDZ-LIM domain protein, at the ribbon synapses in the chicken retina; Wiley-liss, div John Wiley & Sons Inc.; Journal Of Comparative Neurology; 528; 11; 7-2020; 1820-1832
0021-9967
CONICET Digital
CONICET
Autor
Rios, Hugo
Paganelli, Alejandra Raquel
Fosser, Nicolás Sebastián
Resumen
PDLIM's protein family is involved in the rearrangement of the actin cytoskeleton. In the present study, we describe the localization of PDLIM1 in chicken photoreceptors. This study provides evidence that this protein is present at the cone pedicles, as well as in other synapses of the chicken retina. Here, we demonstrate the expression pattern of PDLIM1 through immunofluorescence staining, immunoblots, subcellular fractionation, and immunoprecipitation experiments. Also, we consider the possibility that PDLIM1 may be involved in the synaptic vesicle endocytosis and/or the presynaptic trafficking of synaptic vesicles back to the nonready releasable pool. This endocytotic/exocytotic coupling requires a tight link between exocytic vesicle fusion at defined release sites and endocytic retrieval of synaptic vesicle membranes. In turn, photoreceptor ribbon synaptic structure depends on the cytoskeleton arrangement, both at the active zone-related with exocytosis—as well as at the endocytic zone—periactive zone. To our knowledge, the PDLIM1 protein has not been observed in the pre synapses of the retina. Thus, the present study describes the expression and subcellular localization of PDLIM1 for the first time, as well as its modulation by visual environment in the chicken retina.