info:eu-repo/semantics/article
Bovine Leukemia Virus Presence in Breast Tissue of Argentinian Females and Its Association With Cell Proliferation and Prognosis Markers
Fecha
2018-10Registro en:
Lendez, Pamela Anahí; Martinez Cuesta, Lucia; Nieto Farías, María Victoria; Shen, Hua Min; Dolcini, Guillermina Laura; et al.; Bovine Leukemia Virus Presence in Breast Tissue of Argentinian Females and Its Association With Cell Proliferation and Prognosis Markers; Multidisciplinary Cancer Investigation; Multidisciplinary Cancer Investigation; 2; 10-2018; 16-24
2476-4922
CONICET Digital
CONICET
Autor
Lendez, Pamela Anahí
Martinez Cuesta, Lucia
Nieto Farías, María Victoria
Shen, Hua Min
Dolcini, Guillermina Laura
Buehring, Gertrude Case
Ceriani, Maria Carolina
Resumen
Introduction: Bovine leukemia virus (BLV) causes enzootic bovine leukemia, andis closely related to human T-lymphotropic virus type 1. It expresses microRNAs ofunknown function and codes Tax, the protein that mediates malignant transformation.BLV is capable of infecting B- and T-lymphocytes, endothelial cells, and mammaryepithelial cells of cattle. Several studies demonstrated the presence of BLV DNA inhuman tissue, and it is significantly associated with breast cancer in case-control studiesusing in situ PCR, a highly sensitive and specific technique. The current study was thefirst report of BLV DNA detection by in situ PCR in tissue from Argentinian femaleswith a diagnosis of breast cancer.Methods: In situ PCR was done to detect BLV DNA in 85 human FFPE breast cancertissue samples. The association of BLV DNA and expression of Ki67 and Her-2 wasassessed.Results: BLV DNA presence could be determined in 22.6% of the analyzed samples, andits presence was associated with an increase of the expression and prognosis markers Ki67(P=0.009) and HER-2 (P=0.044) determined by conventional immunohistochemistry.No statistical significance was observed between the presence or absence of hormonalreceptors and the presence of BLV DNA.Conclusions: The obtained results support the idea that BLV might play a role inmalignant tissue transformation.