info:eu-repo/semantics/article
Suspension-Vero cell cultures as a platform for viral vaccine production
Fecha
2009-10Registro en:
Paillet, Cristian; Forno, Angela Guillermina; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Suspension-Vero cell cultures as a platform for viral vaccine production; Elsevier; Vaccine; 27; 46; 10-2009; 6464-6467
0264-410X
CONICET Digital
CONICET
Autor
Paillet, Cristian
Forno, Angela Guillermina
Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
Resumen
Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.