Tesis
Ação da aplicação intraluminal de glutamina e n-acetilcisteína no estresse oxidativo do tecido intestinal durante isquemia e reperfusão hepática : estudo experimental em ratos
Fecha
2014-12-15Registro en:
ARAÚJO FILHO, Adalzízio Vieira de. Ação da aplicação intraluminal de glutamina e n-acetilcisteína no estresse oxidativo do tecido intestinal durante isquemia e reperfusão hepática: estudo experimental em ratos. 2014. 105 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014.
Autor
Caporossi, Cervantes
http://lattes.cnpq.br/9507253213103285
Caporossi, Cervantes
108.334.471-49
http://lattes.cnpq.br/9507253213103285
Nascimento, José Eduardo de Aguilar Siqueira do
142.196.031-15
http://lattes.cnpq.br/0439577158192249
108.334.471-49
Herman, Paulo
064.985.658-96
http://lattes.cnpq.br/7428163118766273
Institución
Resumen
Tissue ischemia followed by reperfusion leads to a series of organic
disorders known by ischemia and reperfusion injury , wich are determined by the production of
reactive oxygen species. These reactive oxygen species lead to depletion of antioxidant
physiological mechanisms and damaging events known as oxidative stress. To reduce such
effects, substances with antioxidant activity come with great therapeutic potential. Among them
stand out glutamine and n-acetylcysteine. Objective: Analyze the effect of enteral glutamine and
n-acetylcysteine injection on oxidative stress caused by ischemia and reperfusion injury intestinal
in rats. Method: Male Wistar rats (n=40) were operated for making closed loop in the small
intestine, followed by hepatic hilar clamping for 10 minutes and then 5 minutes of reperfusion. In
the small intestine closed loop was injected glutamine (glutamine group, n=10), n-acetylcysteine
(n-acetylcysteine group, n=10) or distilled water (control group, n=10). Was not conducted
ischemia and reperfusion injury or injection of any substance in ten animals (sham group). After
reperfusion, has been realized surgical resection of the intestinal closed loop for dosing oxidative
stress markers directly in intestinal tissue (malondialdehyde, reduced glutathione, total
antioxidant capacity, carbonyls protein, myeloperoxidase, reductase glutathione and peroxidase
glutathione). Results: Markers of oxidative injury with representation of gravity by rising values
(malondialdehyde and carbonyls protein ) showned lower values in glutamine and n n-
acetylcysteine groups compared to control group, respectively, 0,0305 vs. 0,094; and 0,0449 vs.
0,094 (p<0,05). About markers whose gravity was representaded by decrease in values (total
antioxidant capacity, reductase glutathione and peroxidase glutathione), the evaluation showed
increased values on glutamine and n-acetylscysteine groups compared with the control group,
respectively, 13,6143 vs. 5,9712 and 11,7021 vs. 5,9712 to total antioxidant capacity; 6,714 vs.
2,8482 and 5,5771 vs. 2,8482 to reductase glutathione; 4,541 vs. 2,1201 and 3,4040 vs. 2,1201 to
peroxidase glutathione (p<0,05). There was exception for reduced glutathione, whose
XVIII
concentrations were higher only in n-acetylcysteine group (p<0,05). Was not identified
myeloperoxidase activity. Conclusion: The enteral injection of glutamine or n-acetylcisteine is
related to a intestinal tissue protection during isquemia/reperfusion, because it determines
lower cellular structure damages, evaluated for its antioxidant capacity.