Tesis
Correlação de citocinas com marcadores biológicos no infarto agudo do miocárdio
Fecha
2015-05-22Registro en:
RIBEIRO, Larissa Nascimento. Correlação de citocinas com marcadores biológicos no infarto agudo do miocárdio. 2015. 116 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2015.
Autor
França, Eduardo Luzia
http://lattes.cnpq.br/3786649407711566
França, Eduardo Luzía
536.319.276-49
http://lattes.cnpq.br/3786649407711566
Scherer, Edson Fredulin
650.563.290-53
http://lattes.cnpq.br/8648967972975593
536.319.276-49
Lima, Victor Vitorino
005.356.961-08
http://lattes.cnpq.br/7503102443670435
Institución
Resumen
Cardiovascular diseases (CVDs) are the main noncommunicable diseases. Among them
is coronary artery disease with a high prevalence today, with acute myocardial
infarction (AMI) an ascension event of the disease. The IAM is due to insufficient blood
flow caused by atherosclerosis, which leads to a prolonged ischemia. As a result, the
heart muscle cells stimulates an inflammatory response through cytokine, especially
pro-inflammatory (IL-2, IL-4, IL-6, TNF, INFγ). Accordingly, the assumption is that
IL-10 and IL-17 is a regulatory feed-back mechanism for balancing the inflammation
caused by pro-inflammatory cytokines in acute myocardial infarction, and also, that
immune response to develop in reactions cascade of cytokines that may be correlated
with each other, and between cardiac injury, displayed through the levels of cardiac
markers (CPK, CK-MB and troponin). Another hypothesis is that there is a promising
biomarker in patients suspected of AMI and confirmed AMI. This is a case-control
study consisting of 3 groups: AMI, suspected AMI and control. The obtained samples
hematologic, biochemical and immunologic assays were performed. The hematological
parameters were analyzed in automated equipment BC-2800 (Mindray), biochemical
parameters Bio-2000 (BioPlus) by the absorbance values and the serum levels of
cytokines obtained by flow cytometry. The results of flow cytometry were generated
using FCAP Array Software (BD) and statistical analyzes were performed using the
BioEstat 5.0. Hematologic parameters only changed the group suspected AMI, while
biochemical parameters showed elevated predominantly in patients with AMI
confirmed. Cytokines such as IL-2, IL-6 and TNF-α have proved to change the suspect
infarction, whereas in the AMI group were IL-2, IL-4, IL-6, TNF-α, IFN and IL- 17A.
The correlation between cytokines and cardiac markers in AMI group was confirmed, in
particular, TNF-α and INF. While the suspect group stood IL-4 and IL-6. In what
regards the relationship between cytokines in patients with myocardial infarction, the
INF correlated with IL-6, TNF -α and IL-17A. Our results suggest that the presence of
AMI, or even suspected, was able to change the hematological, biochemical and
immunological patterns. And the immune response to pro-inflammatory AMI was
predominantly to reactions occurring cascade of cytokines and that these are related to
the size of cardiac injury, especially INF. We can also conclude that high levels of IL-6
and TNF-α may represent a risk factor for AMI and that IL-17A does not act as a
regulator of the inflammatory response, but plays a pro-inflammatory nature can be a
promising marker for AMI.