Artículos de revistas
Proteomic identification of heat shock-induced danger signals in a melanoma cell lysate used in dendritic cell-based cancer immunotherapy
Fecha
2018Registro en:
Journal of Immunology Research, Volumen 2018,
23147156
23148861
10.1155/2018/3982942
Autor
González, Fermín E.
Chernobrovkin, Alexey
Pereda, Cristián
García-Salum, Tamara
Tittarelli, Andrés
López, Mercedes N.
Salazar Onfray, Flavio
Roman, A.
Institución
Resumen
Copyright © 2018 Fermín E. González et al. Autologous dendritic cells (DCs) loaded with cancer cell-derived lysates have become a promising tool in cancer immunotherapy. During the last decade, we demonstrated that vaccination of advanced melanoma patients with autologous tumor antigen presenting cells (TAPCells) loaded with an allogeneic heat shock- (HS-) conditioned melanoma cell-derived lysate (called TRIMEL) is able to induce an antitumor immune response associated with a prolonged patient survival. TRIMEL provides not only a broad spectrum of potential melanoma-associated antigens but also danger signals that are crucial in the induction of a committed mature DC phenotype. However, potential changes induced by heat conditioning on the proteome of TRIMEL are still unknown. The identification of newly or differentially expressed proteins under defined stress conditions is relevant for understanding the lysate immunogenicity. Here, we characterized the proteomic profile of TRIMEL in