Dissertação
Efeito toxicogenético do polimorfismo Ala16Val do gene MnSOD em leucócitos expostos in vitro ao metil mercúrio
Fecha
2012-03-19Registro en:
ALGARVE, Thaís Doeler. TOXIGENETIC EFFECT OF ALA16VAL MnSOD GENE POLYMORPHISM
ON LEUCOCYTES IN VITRO EXPOSED TO METHYL MERCURY. 2012. 77 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.
Autor
Algarve, Thaís Doeler
Institución
Resumen
The environmental contamination by methyl mercury (MeHg) is a great health public problem in
some world regions like Amazonia. The MeHg toxic effects seem to be influenced by
environmental and genetic factors. However, there are few studies evaluating the genetic
influence on MeHg toxicity in humans. Therefore, the aim of this present study was to evaluate
the genetic influence of Ala16Val superoxide dismutase manganese dependent gene
polymorphism (Ala16Val-MnSOD) on cytotoxic effects of in vitro human leucocytes exposed to
MeHg. Subjects were selected from 100 individuals genotyped to Ala16Val-MnSOD
polymorphism (26,4±7,3 years) with different genotpypes (AA=08, VV=06 and AV=12) to
perform the in vitro tests. The reactive oxygen species (ROS) production was measured using
2� 7�-dichlorofluorescein diacetate (DCFDA) fluorimetric assay and the cell viability was
measured using the MTT assay were performed in leucocyte samples with the same subjects
exposed and not exposed to MeHg (2,5μM for 6h). The results showed that AA and VVleucocytes
exposed to MeHg did not increase the ROS levels when compared to the cells that
were not exposed. However, the AV-leucocyte MeHg exposure increased the ROS levels. The
cellular viability comparison among different genotypes exposed to MeHg showed lower AAleucocyte
viability when compared to VV-leucocytes, whereas heterozygous cells (AV)
presented intermediary values. This occurred probabibly due to the fact of AA-leucocytes
present a higher basal H2O2 production than other genotypes. The whole of these results
suggests toxicogenetic effects of Ala16Val-SOD polymorphism in human cells exposed to
MeHg.