Artículos de revistas
“Candidatus Neoehrlichia chilensis” sp. nov.: Molecular detection and characterization of a novel Anaplasmataceae in wild rodents from Valdivia, southern Chile
Fecha
2018-04Registro en:
Mueller A, Monti G, Sepulveda P, Otth C, Bittencourt P, Harrus S, et al. "Candidatus Neoehrlichia chilensis" sp nov.: Molecular detection and characterization of a novel Anaplasmataceae in wild rodents from Valdivia, southern Chile. Transboundary And Emerging Diseases [serial on the Internet]. (n.d.), [cited May 9, 2018]; 65(2): 357-362
1865-1674
eISSN: 1865-1682
WOS:000426530100025
ID de PubMed: 29363276
10.1111/tbed.12815
Autor
Muller, A. [Chile. Universidad Austral de Chile. Facultad de Ciencias Veterinarias]
Otth, C. [Chile. Universidad Austral de Chile. Facultad de Medicina]
Sepúlveda, P. [Chile. Universidad Austral de Chile. Facultad de Ciencias Veterinarias]
Monti, G. [Chile. Universidad Austral de Chile. Facultad de Ciencias Veterinarias]
Bittencourt, P. [Chile. Universidad Mayor. Escuela de Medicina Veterinaria]
Nachum Biala, Y. [Israel. The Hebrew University of Jerusalem. Koret School of Veterinary Medicine]
Gutiérrez, R. [Israel. The Hebrew University of Jerusalem. Koret School of Veterinary Medicine]
Harrus, S. [Israel. The Hebrew University of Jerusalem. Koret School of Veterinary Medicine]
Institución
Resumen
This study aimed to screen wild rodents from southern Chile, for the presence of Anaplasmatacea. Spleen samples from 33 wild rodents trapped in Valdivia Province were screened by conventional PCR (cPCR), targeting the Anaplasmataceae 16S rRNA gene (16S). Positive samples were further evaluated, targeting a larger 16S fragment, groEL operon, and gltA gene, followed by sequencing and phylogenetic analysis. Anaplasmataceae DNA was detected in 15% (five of 33) of the tested rodents (Abrothrix sp. [four of five] and Mus musculus [one of five]). Analysis of sequenced products based on the 16S gene revealed high similarity with Ca. Neoehrlichia mikurensis, Ca. Neoehrlichia lotoris and Ca. Neoehrlichia arcana (97.8%-98.6%). A lower similarity was observed with Candidatus Neoehrlichia groEL (89.7%-92%) and gltA (79.5%-79.9%) loci. According to the 16SrRNA, groEL and gltA phylogenetic analyses, two closely related genotypes of Candidatus Neoehrlichia spp. from Chile were observed, which clustered together in a separate clade from other species in this genus. This study suggests the presence of two genotypes of a novel species of Candidatus Neoehrlichia, proposed as Candidatus Neoehrlichia chilensis, circulating in rodents from Chile. This is the first report of Ca. Neoehrlichia species in rodents from America.
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