Artículos de revistas
Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems
Fecha
2015-01-01Registro en:
Reproduction, Fertility and Development, v. 27, n. 7, p. 1082-1087, 2015.
1448-5990
1031-3613
10.1071/RD14022
2-s2.0-84940050594
2-s2.0-84940050594.pdf
Autor
Universidade Estadual Paulista (Unesp)
Dipartimento di Scienze Veterinarie per la Salute, La Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano
Institución
Resumen
The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL-1 human chorionic gonadotrophin (hCG), 1gmL-1 progesterone (P4) and 1gmL-1 oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P<0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P<0.001). The stage of the oestrous cycle did not influence maturation rates.