Artículos de revistas
Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Fecha
2017-01-01Registro en:
Brazilian Archives Of Biology And Technology. Curitiba-parana: Inst Tecnologia Parana, v. 60, 10 p., 2017.
1516-8913
10.1590/1678-4324-2017160425
S1516-89132017000100454
WOS:000435658200001
S1516-89132017000100454.pdf
Autor
Universidade Estadual Paulista (Unesp)
Univ Fed Piaui
Univ Turin
Ist Protez Sostenibile Piante Turin
Institución
Resumen
Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in Sao Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.