Artículos de revistas
Sulfonation and anticoagulant activity of fungal exocellular β-(1→6)-d-glucan (lasiodiplodan)
Carbohydrate Polymers, v. 92, n. 2, p. 1908-1914, 2013.
Universidade Estadual Paulista (UNESP)
Universidade Federal do Paraná (UFPR)
Universidade Federal do Rio de Janeiro (UFRJ)
An exocellular β-(1→6)-d-glucan (lasiodiplodan) produced by a strain of Lasiodiplodia theobromae (MMLR) grown on sucrose was derivatized by sulfonation to promote anticoagulant activity. The structural features of the sulfonated β-(1→6)-d-glucan were investigated by UV-vis, FT-IR and 13C NMR spectroscopy, and the anticoagulant activity was investigated by the classical coagulation assays APTT, PT and TT using heparin as standard. The content of sulfur and degree of substitution of the sulfonated glucan was 11.73% and 0.95, respectively. UV spectroscopy showed a band at 261 nm due to the unsaturated bond formed in the sulfonation reaction. Results of FT-IR and 13C NMR indicated that sulfonyl groups were inserted on the polysaccharide. The sulfonated β-(1→6)-d-glucan presented anticoagulant activity as demonstrated by the increase in dose dependence of APTT and TT, and these actions most likely occurred because of the inserted sulfonate groups on the polysaccharide. The lasiodiplodan did not inhibit the coagulation tests. © 2012 Elsevier Ltd.
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Vasconcelos, Ana Flora D.; Dekker, Robert F.H.; Barbosa, Aneli M.; Carbonero, Elaine R.; Silveira, Joana L.M.; Glauser, Bianca; Pereira, Mariana Sá; Corradi Da Silva, Maria de Lourdes
Sulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose Mendes, Simone Ferreira; dos Santos, Osvaldo; Barbosa, Aneli M.; Vasconcelos, Ana Flora D.; Aranda-Selverio, Gabriel; Monteiro, Nilson K.; Dekker, Robert F. H.; Pereira, Mariana Sa; Tovar, Ana Maria F.; de Souza Mourao, Paulo A.; Corradi da Silva, Maria de Lourdes
Sulfonation and anticoagulant activity of botryosphaeran from Botryosphaeria rhodina MAMB-05 grown on fructose Universidade Estadual Paulista (UNESP); Universidade Estadual de Londrina (UEL); Lakehead Univ; Universidade Federal do Rio de Janeiro (UFRJ) (Elsevier B.V., 2009-10-01)Botryosphaeran (EPS(FRU)), an exopolysaccharide of the beta-(1 -> 3,1 -> 6)-D-glucan type with 31% branching at C-6, is produced by the fungus Botryosphaeria rhodina MAMB-05 when grown on fructose as carbon source. ...