Artículos de revistas
A simple protocol for a low invasive DNA accessing in Stenella longirostris (Cetacea: Delphinidae)
Fecha
2008-08-01Registro en:
Pan-American Journal of Aquatic Sciences, v. 3, n. 2, p. 130-134, 2008.
1809-9009
2-s2.0-48249136922
2-s2.0-48249136922.pdf
0165348738208319
0000-0003-4524-954X
Autor
Universidade Federal do Espírito Santo (UFES)
Universidade Estadual Paulista (Unesp)
ICMBio
Institución
Resumen
The most significant studies about the spinner dolphin (Stenella longirostris) in the Southwestern Atlantic Ocean were conducted in Fernando de Noronha Archipelago, off Northeastern Brazil. The continuity of these studies depends upon the development of non-invasive methods. In this work, we present results from the skin swabbing sampling procedure for this species. We tested the performance of this method for nuclear and mitochondrial DNA analysis, unknown for this population. A total of skin 161 samples were collected during two expeditions. After the contacts the most of the dolphins remained close to the boat. Microsatellites markers and cytochrome b region primers were evaluated and the respective fragments were successfully amplified. Thus, skin swabbing may be considered an efficient strategy to obtain tissue samples for spinner dolphin genetic analysis in Fernando de Noronha Archipelago.