Artículos de revistas
Comparison of a multiplex-PCR assay with mycolic acids analysis and conventional methods for the identification of Mycobacteria
Date
2003-06-02Registration in:
Microbiology and Immunology, v. 47, n. 5, p. 307-312, 2003.
0385-5600
10.1111/j.1348-0421.2003.tb03401.x
WOS:000182726900001
2-s2.0-0038585025
2114570774349859
Author
Faculdade de Medicina de Marília (FAMEMA)
Universidade Estadual Paulista (UNESP)
Centers for Dis. Controls/Prevention
Institutions
Abstract
A fast, sensitive and cost-effective multiplex-PCR assay for Mycobacterium tuberculosis complex (MTC) and Mycobacterium avium (M. avium) identification for routine diagnosis was evaluated. A total of 158 isolates of mycobacteria from 448 clinical specimens from patients with symptoms of mycobacterial disease were analyzed. By conventional biochemical methods 151 isolates were identified as M. tuberculosis, five as M. avium and two as Mycobacterium chelonae (M. chelonae). Mycolic acid patterns confirmed these results. Multiplex-PCR detected only IS6110 in isolates identified as MTC, and IS1245 was found only in the M. avium isolates. The method applied to isolates from two patients, identified by conventional methods and mycolic acid analysis, one as M. avium and other as M. chelonae, resulted positive for IS6110, suggesting co-infection with M. tuberculosis. These patients were successfully submitted to tuberculosis treatment. The multiplex-PCR method may offer expeditious identification of MTC and M. avium, which may minimize risks for active transmission of these organisms and provide useful treatment information.
Subjects
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