Artículos de revistas
Chalcone-induced apoptosis through caspase-dependent intrinsic pathways in human hepatocellular carcinoma cells
Fecha
2016Registro en:
International Journal of Molecular Sciences, Volumen 17, Issue 2, 2018,
14220067
16616596
10.3390/ijms17020260
Autor
Ramirez-Tagle, Rodrigo
Escobar, Carlos A.
Romero, Valentina
Montorfano, Ignacio
Armisén, Ricardo
Borgna, Vincenzo
Jeldes, Emanuel
Pizarro, Luis
Simon, Felipe
Echeverria, Cesar
Institución
Resumen
© 2016 by the authors; licensee MDPI, Basel, Switzerland. Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers worldwide. Chemoprevention of HCC can be achieved through the use of natural or synthetic compounds that reverse, suppress or prevent the development of cancer progression. In this study, we investigated the antiproliferative effects and the mechanism of action of two compounds, 2,3,4′-trimethoxy-2′-hydroxy-chalcone (CH1) and 3′-bromo-3,4-dimethoxy-chalcone (CH2), over human hepatoma cells (HepG2 and Huh-7) and cultured mouse hepatocytes (HepM). Cytotoxic effects were observed over the HepG2 and Huh-7, and no effects were observed over the HepM. For HepG2 cells, treated separately with each chalcone, typical apoptotic laddering and nuclear condensation were observed. Additionally, the caspases and Bcl-2 family proteins activation by using Western blotting and immunocytochemistry were studied. Caspase-8 was not activated, but caspase-3 and -9 were both a