Artículos de revistas
Role of Akt and Ca2+ on cell permeabilization via connexin43 hemichannels induced by metabolic inhibition
Fecha
2015Registro en:
Biochimica et Biophysica Acta - Molecular Basis of Disease, Volumen 1852, Issue 7, 2018, Pages 1268-1277
1879260X
09254439
10.1016/j.bbadis.2015.03.004
Autor
Salas, Daniela
Puebla, Carlos
Lampe, Paul D.
Lavandero González, Sergio
Sáez, Juan Carlos
Institución
Resumen
© 2015 Elsevier B.V. Connexin hemichannels are regulated under physiological and pathological conditions. Metabolic inhibition, a model of ischemia, promotes surface hemichannel activation associated, in part, with increased surface hemichannel levels, but little is known about its underlying mechanism. Here, we investigated the role of Akt on the connexin43 hemichannel's response induced by metabolic inhibition. In HeLa cells stably transfected with rat connexin43 fused to EGFP (HeLa43 cells), metabolic inhibition induced a transient Akt activation necessary to increase the amount of surface connexin43. The increase in levels of surface connexin43 was also found to depend on an intracellular Ca2+ signal increase that was partially mediated by Akt activation. However, the metabolic inhibition-induced Akt activation was not significantly affected by intracellular Ca2+ chelation. The Akt-dependent increase in connexin43 hemichannel activity in HeLa43 cells also occurred after oxygen-gluc