Artículo de revista
Probiotic screening and safety evaluation of lactobacillus strains from plants, artisanal goat cheese, human stools, and breast milk
Fecha
2014Registro en:
Journal of Medicinal Food, Volumen 17, Issue 4, apr 2014
15577600
1096620X
10.1089/jmf.2013.0030
Autor
Gotteland, Martin
Cires, Maria Jose
Carvallo, Claudia
Vega, Natalia
Ramirez, Maria Antonieta
Morales, Pamela
Rivas, Patricia
Astudillo, Fernanda
Navarrete, Paola
Dubos, Céline
Figueroa, Alvaro
Troncoso, Miriam
Ulloa, Carolina
Mizgier, Maria Luisa
Carrasco Pozo, Catalina
Speisky, Hernan
Brunser, Oscar
Figueroa, Guillermo
Institución
Resumen
The aim of this study was to select autochthonous strains of Lactobacillus from stools of healthy infants and adults, human milk, artisanal goat cheese, and fruits and vegetables according to their probiotic properties and safety. From 421 strains of Lactobacillus isolated, 102 (24.2%) were shown to be tolerant to gastric pH and bile salts; they were used to determine their anti-Helicobacter pylori (agar diffusion assay), antioxidant (oxygen radical absorption capacity), and anti-inflammatory (inhibition of interleukin-8 release by tumor necrosis factor-α-stimulated HT-29 cells) activities as well as their ability to adhere to intestinal (Caco-2) and gastric (AGS) epithelial cells. Results obtained were compared with three commercial probiotic Lactobacillus rhamnosus GG, L. plantarum 299v, and L. johnsonii NCC533. The five strains most efficient according to these activities were subsequently identified by sequencing their 16S rRNA gene, their susceptibility to antibiotics was determin