dc.creator | Depix, María S. | |
dc.creator | Martínez, Jorge | |
dc.creator | Santibañez, Francisco | |
dc.creator | Rovirosa, Juana | |
dc.creator | San Martín Barrientos, Aurelio | |
dc.creator | Maccioni Baraona, Ricardo | |
dc.date.accessioned | 2019-01-29T17:15:46Z | |
dc.date.available | 2019-01-29T17:15:46Z | |
dc.date.created | 2019-01-29T17:15:46Z | |
dc.date.issued | 1998 | |
dc.identifier | Molecular and Cellular Biochemistry, Volumen 187, Issue 1-2, 2018, Pages 191-199 | |
dc.identifier | 03008177 | |
dc.identifier | 10.1023/A:1006879308861 | |
dc.identifier | https://repositorio.uchile.cl/handle/2250/163324 | |
dc.description.abstract | We investigated the effects of the drug 14-keto-stypodiol diacetate (SDA) extracted from the seaweed product Stypopodium flabelliforme, in inhibiting the cell growth and tumor invasive behavior of DU-145 human prostate cells. In addition, the molecular action of the drug on microtubule assembly was analyzed. The effects of this diterpenoid drug in cell proliferation of DU-145 tumor cells in culture revealed that SDA at concentrations of 5 μM decreased cell growth by 14% while at 45 μM a 61% decrease was found, as compared with control cells incubated with the solvent but in the absence of the drug. To study their effects on the cell cycle, DU-145 cells were incubated with increasing concentrations of SDA and the distribution of cell-cycle stages was analyzed by flow cytometry. Interestingly, the data showed that 14-keto-stypodiol diacetate dramatically increased the proportion of cells in the G2/M phases, and decreased the number of cells at the S phase of mitosis, as compared with app | |
dc.language | en | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
dc.source | Molecular and Cellular Biochemistry | |
dc.subject | 14-keto-stypodiol diacetate | |
dc.subject | Cell proliferation | |
dc.subject | DU-145 cell line | |
dc.subject | Microtubule assembly | |
dc.subject | Plasminogen activator (u-PA) | |
dc.subject | Stypopodium flabelliforme | |
dc.subject | Tumor invasiveness | |
dc.title | The compound 14-keto stypodiol diacetate from the algae Stypopodium flabelliforme inhibits microtubules and cell proliferation in DU-145 human prostatic cells | |
dc.type | Artículo de revista | |