Artículo de revista
Immature rat ovaries become revascularized rapidly after autotransplantation and show a gonadotropin-dependent increase in angiogenic factor gene expression
Endocrinology, Volumen 134, Issue 3, 2018, Pages 1146-1154
Dissen, G. A.
Lara Peñaloza, Hernán
Fahrenbach, W. H.
Costa, M. E.
Ojeda, S. R.
When the ovaries of 23-day-old juvenile rats are transplanted to an ectopic site, they recover within 1 week the ability to control gonadotropin secretion via steroid negative feedback. Vascular corrosion casting followed by scanning electron microscopy revealed that the transplanted ovary becomes profusely revascularized within 48 h after transplantation. Vascular ingrowth was accompanied by a 40- to 60-fold increase in expression of the genes encoding two angiogenic factors, vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGFβ1), as assessed by RNA blot hybridization of the corresponding mRNAs. Although TGFβ3 mRNA levels also increased, no changes in the levels of mRNAs encoding other putative angiogenic factors, such as TGFα, basic fibroblast growth factor, and TGFβ2, were observed. Hybridization histochemistry demonstrated that in intact ovaries, VEGF mRNA is mainly expressed in granulosa cells of the cumulus oophorus and thecal cells of large antral fo