Aminoacyl transfer from phenylalanyl-tRNA microinjected into xenopus laevis oocytes

dc.creatorGatica, Marta
dc.creatorAllende, Jorge E.
dc.date.accessioned2019-01-29T15:45:59Z
dc.date.available2019-01-29T15:45:59Z
dc.date.created2019-01-29T15:45:59Z
dc.date.issued1977
dc.identifierBiochemical and Biophysical Research Communications, Volumen 79, Issue 2, 2018, Pages 352-356
dc.identifier10902104
dc.identifier0006291X
dc.identifier10.1016/0006-291X(77)90164-4
dc.identifierhttps://repositorio.uchile.cl/handle/2250/162297
dc.description.abstractXenopus laevis oocytes were injected with [14C] phe-tRNA and the fate of the aminoacyl moiety was studied. The radioactive phenylalanine is gradually hydrolized off the tRNA once inside the cell. The rate of deacylation of the tRNA is not affected by inhibition of cellular protein synthesis by puromycin or cycloheximide. Part of the radioactive amino acid that leaves the tRNA (30 to 65%) is transferred directly into the oocyte nascent proteins as evidenced by the fact that its incorporation into proteins is not reduced by coinjection with a large excess of [12C] phenylalanine. Aminoacyl transfer from injected phe-tRNA into proteins is inhibited by puromycin and cycloheximide. © 1977.
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceBiochemical and Biophysical Research Communications
dc.subjectBiophysics
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.titleAminoacyl transfer from phenylalanyl-tRNA microinjected into xenopus laevis oocytes
dc.typeArtículo de revista


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