Artículos de revistas
Pure platelet-rich plasma and supernatant of calcium-activated P-PRP induce different phenotypes of human macrophages
Fecha
2018Registro en:
Regen.Med. (2018) 13(4), 427–441
10.2217/rme-2017-0122
Autor
Escobar, Gisselle
Escobar Álvarez, Alejandro
Ascui, Gabriel
Tempio, Fabián I.
Ortiz, María C.
Pérez Núñez, Claudio
López, Mercedes N.
Institución
Resumen
Aim: This study aimed to evaluate the effect of two platelet preparations used in the clinic, pure platelet-rich plasma (P-PRP) and the supernatant of calcium-activated P-PRP (S-PRP), on the phenotype of human macrophages. Materials & methods: Surface markers and cytokine production of human monocyte-derived macrophages were analyzed after 24h stimulation with P-PRP or S-PRP. Results: P-PRP and S-PRP present no difference in the expression of CD206, a M2 tissue-repair macrophage-related marker. However, these same macrophages presented different levels of CD163, CD86 as well as different IL-10 secretion capacities after 24h incubation. Conclusion: These platelet preparations do not have an equivalent biological effect over macrophages, which suggest that they may present different clinical regenerative potentials.