Artículos de revistas
Analytical parameters of the microplate-based ORAC pyrogallol red assay
Fecha
2011Registro en:
Journal of AOAC International, Volumen 94, Issue 5, 2018, Pages 1562-1566
10603271
10.5740/jaoacint.11-033
Autor
Ortiz, Rocío
Antilén, Mónica
Speisky Cosoy, Hernán
Aliaga, Margarita E.
López Alarcón, Camilo
Institución
Resumen
The analytical parameters of the microplatebased oxygen radicals absorbance capacity (ORAC) method using pyrogallol red (PGR) as probe (ORAC-PGR) are presented. In addition, the antioxidant capacity of commercial beverages, such as wines, fruit juices, and iced teas, is estimated. A good linearity of the area under the curve (AUC) versus Trolox concentration plots was obtained [AUC = (845 ± 110) + (23 ± 2) [Trolox, μM], R = 0.9961, n = 19]. QC experiments showed better precision and accuracy at the highest Trolox concentration (40 μM) with RSD and REC (recuperation) values of 1.7 and 101.0%, respectively. When red wine was used as sample, the method also showed good linearity [AUC = (787 ± 77) + (690 ± 60) [red wine, μL/mL]; R = 0.9926, n = 17], precision and accuracy with RSD values from 1.4 to 8.3%, and REC values that ranged from 89.7 to 103.8%. Additivity assays using solutions containing gallic acid and Trolox (or red wine) showed an additive protection of PGR given by the samples